Determination of Total Chlorophyll, Carbohydrate and Hydrolyzing Enzymes Contents in Mitragyna Parvifolia Leaves


  • Om Prakash Meena Government College Rajgarh, Alwar, India



Histochemistry, metabolites, galls


Histochemical studies have shown that gall-inducing agents can alter the cell and tissue metabolism on the host plant part. Morphological changes in the host tissues are ulti-mately linked with physiological changes. Histochemistry enables the localization of various metabolites present in the tissue. To understand the morphological changes in terms of physiology certain histochemical studies have been carried out. An attempt has been made to interpret the biochemical data in terms of cells, tissues and tissue systems. Fresh hand-cut sections were used to study the changes brought about in the distribu-tion of various metabolites and enzymes of the normal and gall tissues. The metabolites studied during the present investigation were chlorophyll, totally insoluble polysaccha-rides and enzymes.


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P. Prade, R. Diaz, M. D. Vitorino, et al., “Galls induced by Calophyalatiforceps (Hemiptera: Calophyidae) reduce leaf perfor-mance and growth of Brazilian peppertree,” Biocontrol Science and Technology, vol.26, no.1, pp.23-34, 2016.

M. Y. Huang, H. M. Chou, Y. T. Chang, et al., “The number of cecidomyiid insect galls affects the photosynthesis of Machi-lusthunbergii host leaves,” Journal of Asia-Pacific Entomology, vol.17, no.2, pp.151-154, 2014.

W. L. Gonzáles, P. P. Caballero & R. Medel, “Galler-induced reduction of shoot growth and fruit production in the shrub Col-liguajaintegerrima (Euphorbiaceae),” Revista Chilena de Historia Natural, vol.78, no.3, pp.393-399, 2005.

V. K. Macioszek, M. Wielanek, L. Morkunas, et al., “Leaf position‐dependent effect of Alternariabrassicicola development on host cell death, photosynthesis and secondary metabolites in Brassica juncea,” Physiologia Plantarum, vol.168, no.3, pp.601-616, 2020.

D. I. Arnon, “Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris,” Plant Physiol, vol.24, no.1, pp.1-15, 1949.

A. Maretzki, M. Thom & L. G. Niekell, “Utilization and metabolism of carbohydrates in cell and callus cultures in tissue Cul-ture and Plant Science,” Acad Press. London, pp.329-362, 1974.

T. Kosuge & J. A. Kimpel, “Energy use and metabolic regulator in plant pathogen interactions in Effects of disease on the physiology and growth of the plants,” (Ed.) P. G. Aynes Cambridge Univ. Press, Cambridge, pp. 29-45, 1981.

H. E. Street & A. Goldsworthy, “Effect of organized and unorganized systems in plant physiology Studies on carbohydrate metabolism of excised roots,” F.C. Steward 3B, vol.3, no.244, 1969.

A. Srivastava, “Studies on fungal induced galls of Coriandrumsativum caused by Protomycesmacrosporusin vivo and in vitro,” Ph.D. Thesis University of Rajasthan Jaipur India, 1992.

N. S. Shekhawat, K. G. Ramawat, H. C. Arya, “Carbohydrate protein, phenols and enzymes (PPO, PRO & 1AA oxidase) in gall and normal tissues of Achyranthesaspera,” Linn. Curr. Sci., vol.47, pp.780-781, 1978.

P. Tandon, H. C. Arya, “Effect growth regulations on carbohydrate metabolism of Zizyphusjujuba gall and normal stem tissue in culture,” Biochem Physiol flanzen, vol.174, no.9, pp.772-779, 1979.

A. Karnawat, “Studies on insect induced galls of some fruit trees,” Ph. D. Thesis University of Rajasthan Jaipur, 1988.

A. Kumar,” Physiology and host-parasite interaction of insect induced galls of Prosopiscinerariain vivo and in vitro,” Ph. D. Thesis University of Rajasthan Jaipur, 1996.

M. Mathur, “Studies on insect induced galls of certain economically important tree species,” Ph.D. Thesis, University of Rajasthan Jaipur, 2002.

G. L. Miller, “Use of dinitrosalicylic acid reagent for determination of reducing sugar,” Analyst Chem, vol.31, pp.426-428, 1972.

M. Dubois, K. A. Gilles, J. K. Hamilton, et al., “Colorimetric determination of sugars and related substances,” Analyst Chem, vol.28, no.3, pp.350-356, 1951.





How to Cite

Om Prakash Meena, “Determination of Total Chlorophyll, Carbohydrate and Hydrolyzing Enzymes Contents in Mitragyna Parvifolia Leaves”, J. Appl. Sci. Educ., vol. 3, no. 1, pp. 1–6, Apr. 2023.




Research Article